Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
NACHT, LRR and PYD domains-containing protein 3 (NLRP3) is a ~118 kDa protein. Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis. In response to pathogens and other damage-associated signals that affect the integrity of membranes, initiates the formation of the inflammasome polymeric complex composed of NLRP3, CASP1 and PYCARD/ASC. Recruitment of pro-caspase-1 (proCASP1) to the NLRP3 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion and pyroptosis. Activation of NLRP3 inflammasome is also required for HMGB1 secretion; stimulating inflammatory responses. Involved in the homeostatic wound healing response to tissue injury, a multistep cascade that guides neutrophil migration to necrotic sites while avoiding collateral damage of healthy tissues.
1. Martinon, F. et al. (2006) Nature 440, 237-41. PMID: 16407889
2. Dostert, C. et al. (2008) Science (New York, N.Y.) 320, 674-7. PMID: 18403674
3. Hornung, V. et al. (2008) Nature immunology 9, 847-56. PMID: 18604214
4. Subramanian, N. et al. (2013) Cell 153, 348-61. PMID: 23582325
5. Coll, RC. et al. (2015) Nature medicine 21, 248-55. PMID: 25686105
6. Song, H. et al. (2016) Nature communications 7, 13727. PMID: 27929086
