Recombinant Human NLRP3 Protein, N-His (PX-P5856) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
Q96P20
蛋白长度
Met1-Arg157
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
20.97 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
NLRP3, NACHT, LRR and PYD domains-containing protein 3, Caterpiller protein 1.1, C1orf7, PYPAF1, PYRIN-containing APAF1-like protein 1, CIAS1, CLR1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, Angiotensin/vasopressin receptor AII/AVP-like, NALP3
背景
NACHT, LRR and PYD domains-containing protein 3 (NLRP3) is a ~118 kDa protein. Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis. In response to pathogens and other damage-associated signals that affect the integrity of membranes, initiates the formation of the inflammasome polymeric complex composed of NLRP3, CASP1 and PYCARD/ASC. Recruitment of pro-caspase-1 (proCASP1) to the NLRP3 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion and pyroptosis. Activation of NLRP3 inflammasome is also required for HMGB1 secretion; stimulating inflammatory responses. Involved in the homeostatic wound healing response to tissue injury, a multistep cascade that guides neutrophil migration to necrotic sites while avoiding collateral damage of healthy tissues.
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3. Hornung, V. et al. (2008) Nature immunology 9, 847-56. PMID: 18604214
4. Subramanian, N. et al. (2013) Cell 153, 348-61. PMID: 23582325
5. Coll, RC. et al. (2015) Nature medicine 21, 248-55. PMID: 25686105
6. Song, H. et al. (2016) Nature communications 7, 13727. PMID: 27929086
