Anti-LAMTOR3 Polyclonal Antibody (HV209014) is a rabbit polyclonal antibody detecting LAMTOR3 in ELISA, IHC, WB. Suitable for Bovine, Danio rerio, Chicken, Human, and Mouse.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human LAMTOR3 (Met1-Ser124).
靶标
Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3, Mitogen-activated protein kinase kinase 1-interacting protein 1, Ragulator complex protein LAMTOR3, LAMTOR3, MAP2K1IP1, MAPKSP1, MEK-binding partner 1, Mitogen-activated protein kinase scaffold protein 1, Mp1
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Ragulator complex protein LAMTOR3 is a ~13 kDa protein. As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
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7. Shen, K. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 9545-9550. PMID: 30181260
