Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014)

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概述
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参考文献
产品说明书

概述

货号

VK577014

品牌

abinScience

描述

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014) is a rabbit polyclonal antibody detecting ORF3a protein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant SARS-CoV-2 ORF3a (Leu115-Leu275).

靶标

ORF3a protein, ORF3a, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, 3a

纯化方式

Purified by antigen affinity column.

Accession号

P0DTC3

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

ORF3a protein is a ~31 kDa protein. Plays a role in viral egress via lysosomal trafficking. Forms homotetrameric ion channels (viroporins) localized at endosomes and lysosomes, that may induce deacidification of lysosomes, allowing safe egress of virions via lysosomal trafficking. Also blocks autolysosome formation by binding and sequestering the host component VPS39 for homotypic fusion and protein sorting (HOPS) on late endosomes. This prevents fusion of autophagosomes with lysosomes, disrupting autophagy and facilitating virus egress. Induces host RETREG1/FAM134B-dependent reticulophagy by interacting with host HMGB1 and enhancing the association between HMGB1 and host BECN1.

1. Ghosh, S. et al. (2020) Cell 183, 1520-1535.e14. PMID: 33157038

2. Miao, G. et al. (2021) Developmental cell 56, 427-442.e5. PMID: 33422265

3. Kern, DM. et al. (2021) Nature structural & molecular biology 28, 573-582. PMID: 34158638

4. Zhang, X. et al. (2022) Autophagy 18, 2576-2592. PMID: 35239449

Note

For research use only.

图片

参考文献

产品说明书

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014)

概述

图片

参考文献

产品说明书

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