Recombinant SARS-CoV-2 ORF3a Protein, N-His (PX-COV-P063) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
P0DTC3
蛋白长度
Leu115-Leu275
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
20.79 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
ORF3a protein, ORF3a, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, 3a
背景
ORF3a protein is a ~31 kDa protein. Plays a role in viral egress via lysosomal trafficking. Forms homotetrameric ion channels (viroporins) localized at endosomes and lysosomes, that may induce deacidification of lysosomes, allowing safe egress of virions via lysosomal trafficking. Also blocks autolysosome formation by binding and sequestering the host component VPS39 for homotypic fusion and protein sorting (HOPS) on late endosomes. This prevents fusion of autophagosomes with lysosomes, disrupting autophagy and facilitating virus egress. Induces host RETREG1/FAM134B-dependent reticulophagy by interacting with host HMGB1 and enhancing the association between HMGB1 and host BECN1.
1. Ghosh, S. et al. (2020) Cell 183, 1520-1535.e14. PMID: 33157038
2. Miao, G. et al. (2021) Developmental cell 56, 427-442.e5. PMID: 33422265
