Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

JN055014

品牌

abinScience

描述

Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014) is a rabbit polyclonal antibody detecting Toxin A in ELISA, IHC, WB. Suitable for Clostridium novyi.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Clostridium novyi

应用

ELISA, IHC, WB

同种型

IgG

免疫原

E. coli - derived recombinant Clostridium novyi Toxin A/tcdA (Met1-Glu551).

靶标

Toxin A, 3.4.22.-, N-acetylglucosaminyltransferase TcdA, 2.4.1.-, tcdA, toxA

纯化方式

Purified by antigen affinity column.

Accession号

Q46149

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Toxin A (tcdA) is a ~250 kDa protein. Precursor of a cytotoxin, which enters into host cells and mediates autoprocessing to release the active toxin (N-acetylglucosaminyltransferase TcdA) into the host cytosol. Once entered into host cells, acidification in the endosome promotes the membrane insertion of the translocation region and formation of a pore, leading to translocation of the GT44 and peptidase C80 domains across the endosomal membrane. This activates the peptidase C80 domain and autocatalytic processing, releasing the N-terminal part (N-acetylglucosaminyltransferase TcdA), which constitutes the active part of the toxin, in the cytosol.

_x005F_x005F_x000D_ _x005F_x005F_x000D_

1. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

_x005F_x005F_x000D_

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-TCDA polyclonal antibody at 1 ug/mL on various samples.
Lane 1: JN055012 100ng

参考文献

产品说明书

Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014)

概述

图片

参考文献

产品说明书

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