Anti-Human IFT20 Polyclonal Antibody (HP142014)

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概述
图片
参考文献
产品说明书

概述

货号

HP142014

品牌

abinScience

描述

Anti-Human IFT20 Polyclonal Antibody (HP142014) is a rabbit polyclonal antibody detecting IFT20 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human IFT20 (Met1-Lys132).

靶标

hIFT20, IFT20, Intraflagellar transport protein 20 homolog

纯化方式

Purified by antigen affinity column.

Accession号

Q8IY31

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Intraflagellar transport protein 20 homolog (IFT20) is a ~15 kDa protein. Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Regulates the platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling. Essential for male fertility.

1. Pugacheva, EN. et al. (2007) Cell 129, 1351-63. PMID: 17604723

2. Follit, JA. et al. (2006) Molecular biology of the cell 17, 3781-92. PMID: 16775004

3. Schmid, FM. et al. (2018) The Journal of cell biology 217, 151-161. PMID: 29237719

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human IFT20 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IFT20 Polyclonal Antibody (HP142014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IFT20 Polyclonal Antibody (HP142014)

概述

图片

参考文献

产品说明书

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