Anti-Human ELAVL1 Polyclonal Antibody (HS864014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HS864014

品牌

abinScience

描述

Anti-Human ELAVL1 Polyclonal Antibody (HS864014) is a rabbit polyclonal antibody detecting ELAVL1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Xenopus laevis, and Xenopus tropicalis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human ELAVL1 (Gly18-Gln198).

靶标

ELAV-like protein 1, HuR, ELAVL1, Hu-antigen R, HUR

纯化方式

Purified by antigen affinity column.

Accession号

Q15717

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

ELAV-like protein 1 (ELAVL1) is a ~36 kDa protein. RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation. Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs.

1. Tran, H. et al. (2003) Molecular and cellular biology 23, 7177-88. PMID: 14517288

2. Doller, A. et al. (2008) Molecular and cellular biology 28, 2608-25. PMID: 18285462

3. Chen, X. et al. (2019) Nature cell biology 21, 978-990. PMID: 31358969

4. Zhu, S. et al. (2020) Nature communications 11, 1685. PMID: 32245947

5. Tran, H. et al. (2004) Molecular cell 13, 101-11. PMID: 14731398

6. Meisner, NC. et al. (2007) Nature chemical biology 3, 508-15. PMID: 17632515

7. Wang, H. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 373-80. PMID: 23519412

8. Ma, WJ. et al. (1996) The Journal of biological chemistry 271, 8144-51. PMID: 8626503

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ELAVL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: A549 cell lysate
Lane 3: K562 cell lysate
Lane 4: H1299 cell lysate
Western blot

Western blot analysis was performed using anti-ELAVL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lane 4: A549 cell lysate
Lane 5: N2A cell lysate
Lane 6: K562 cell lysate
Lane 7: H1299 cell lysate

参考文献

产品说明书

Anti-Human ELAVL1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ELAVL1 Polyclonal Antibody (HS864014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ELAVL1 Polyclonal Antibody (HS864014)

概述

图片

参考文献

产品说明书

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