Anti-Human H4C1 Polyclonal Antibody (HX879014) is a rabbit polyclonal antibody detecting H4C1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Acropora formosa, and Agaricus bisporus.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human H4C1 (Asn26-Gly103).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Histone H4 (H4C1) is a ~11 kDa protein. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-H4C1 polyclonal antibody at 1ug/mL on various samples. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF-7 cell lysate Lane 4: 293T cell lysate Lane 5: HL-60 cell lysate Lane 6: Raji cell lysate
