Anti-Human SIRT5 Polyclonal Antibody (HP532014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HP532014

品牌

abinScience

描述

Anti-Human SIRT5 Polyclonal Antibody (HP532014) is a rabbit polyclonal antibody detecting SIRT5 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human SIRT5 (Met40-Ser310).

靶标

SIR2-like protein 5, SIRT5, SIR2L5, Regulatory protein SIR2 homolog 5, NAD-dependent protein deacylase sirtuin-5, mitochondrial

纯化方式

Purified by antigen affinity column.

Accession号

Q9NXA8

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

NAD-dependent protein deacylase sirtuin-5, mitochondrial (SIRT5) is a ~33 kDa protein. NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2.

1. Peng, C. et al. (2011) Molecular & cellular proteomics : MCP 10, M111.012658. PMID: 21908771

2. Du, J. et al. (2011) Science (New York, N.Y.) 334, 806-9. PMID: 22076378

3. Tan, M. et al. (2014) Cell metabolism 19, 605-17. PMID: 24703693

4. Yang, X. et al. (2018) Cancer research 78, 372-386. PMID: 29180469

5. Lin, ZF. et al. (2013) Biochemical and biophysical research communications 441, 191-5. PMID: 24140062

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human SIRT5 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SIRT5 Polyclonal Antibody (HP532014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SIRT5 Polyclonal Antibody (HP532014)

概述

图片

参考文献

产品说明书

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