Anti-Human IST1 Polyclonal Antibody (HX152014)

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100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HX152014

品牌

abinScience

描述

Anti-Human IST1 Polyclonal Antibody (HX152014) is a rabbit polyclonal antibody detecting IST1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human IST1 (Met1-Glu186).

靶标

Putative MAPK-activating protein PM28, IST1, IST1 homolog, KIAA0174, hIST1

纯化方式

Purified by antigen affinity column.

Accession号

P53990

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

IST1 homolog is a ~39 kDa protein. ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation. Is required for efficient abscission during cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2.

1. Bajorek, M. et al. (2009) Molecular biology of the cell 20, 1360-73. PMID: 19129479

2. Vietri, M. et al. (2015) Nature 522, 231-5. PMID: 26040712

3. Gu, M. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E2166-E2175. PMID: 28242692

4. Agromayor, M. et al. (2009) Molecular biology of the cell 20, 1374-87. PMID: 19129480

5. Allison, R. et al. (2013) The Journal of cell biology 202, 527-43. PMID: 23897888

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human IST1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IST1 Polyclonal Antibody (HX152014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IST1 Polyclonal Antibody (HX152014)

概述

图片

参考文献

产品说明书

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