Anti-Human PHLPP1 Polyclonal Antibody (HT225014)

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概述
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参考文献
产品说明书

概述

货号

HT225014

品牌

abinScience

描述

Anti-Human PHLPP1 Polyclonal Antibody (HT225014) is a rabbit polyclonal antibody detecting PHLPP1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human PHLPP1 (Ser1168-Thr1425).

靶标

KIAA0606, Pleckstrin homology domain-containing family E member 1, hSCOP, PHLPP, SCOP, PHLPP1, PH domain leucine-rich repeat-containing protein phosphatase 1, PLEKHE1, Suprachiasmatic nucleus circadian oscillatory protein, PH domain-containing family E member 1

纯化方式

Purified by antigen affinity column.

Accession号

O60346

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

PH domain leucine-rich repeat-containing protein phosphatase 1 (PHLPP1) is a ~184 kDa protein. Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth.

1. Gao, T. et al. (2005) Molecular cell 18, 13-24. PMID: 15808505

2. Brognard, J. et al. (2007) Molecular cell 25, 917-31. PMID: 17386267

3. Gao, T. et al. (2008) The Journal of biological chemistry 283, 6300-11. PMID: 18162466

4. Qiao, M. et al. (2010) Molecular cell 38, 512-23. PMID: 20513427

5. Liu, J. et al. (2011) Molecular and cellular biology 31, 4917-27. PMID: 21986499

6. Liu, J. et al. (2009) Oncogene 28, 994-1004. PMID: 19079341

7. Li, X. et al. (2014) Gastroenterology 146, 1301-12.e1-10. PMID: 24530606

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human PHLPP1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PHLPP1 Polyclonal Antibody (HT225014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PHLPP1 Polyclonal Antibody (HT225014)

概述

图片

参考文献

产品说明书

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