Anti-Human PPP2CA Polyclonal Antibody (HX136014) is a rabbit polyclonal antibody detecting PPP2CA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Human PPP2CA (Met1-Leu309).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (PPP2CA) is a ~35 kDa protein. Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events. PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate various proteins, such as SV40 large T antigen, AXIN1, p53/TP53, PIM3, WEE1.
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2. Losman, JA. et al. (2003) The Journal of biological chemistry 278, 4800-5. PMID: 12473674
3. Li, HH. et al. (2007) The EMBO journal 26, 402-11. PMID: 17245430
4. Watkins, GR. et al. (2012) The Journal of biological chemistry 287, 24207-15. PMID: 22613722
5. Zheng, H. et al. (2020) Science (New York, N.Y.) 370. PMID: 33243860
