Anti-Human CHCHD3 Polyclonal Antibody (HP686014)

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100ug

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概述
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产品说明书

概述

货号

HP686014

品牌

abinScience

描述

Anti-Human CHCHD3 Polyclonal Antibody (HP686014) is a rabbit polyclonal antibody detecting CHCHD3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human CHCHD3 (Ser58-Gly227).

靶标

MICOS complex subunit MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, MINOS3, MIC19, CHCHD3

纯化方式

Purified by antigen affinity column.

Accession号

Q9NX63

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

MICOS complex subunit MIC19 (CHCHD3) is a ~26 kDa protein. Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription.

1. Ott, C. et al. (2015) PloS one 10, e0120213. PMID: 25781180

2. Stephan, T. et al. (2020) The EMBO journal 39, e104105. PMID: 32567732

3. Hu, C. et al. (2020) Cell death & disease 11, 940. PMID: 33130824

4. Liu, H. et al. (2012) PloS one 7, e34832. PMID: 22567091

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-CHCHD3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Hepg2 cell lysate
Lane 5: A549 cell lysate
Lane 6: 293T cell lysate
Lane 7: Mouse kidney lysate
Lane 8: Mouse liver lysate
Lane 9: Mouse heart lysate
Lane 10: Rat kidney lysate
Lane11: Rat Liver lysate
Lane12: Rat heart lysate

参考文献

产品说明书

Anti-Human CHCHD3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CHCHD3 Polyclonal Antibody (HP686014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CHCHD3 Polyclonal Antibody (HP686014)

概述

图片

参考文献

产品说明书

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