Anti-Human DDX1 Polyclonal Antibody (HP260014)

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概述
图片
参考文献
产品说明书

概述

货号

HP260014

品牌

abinScience

描述

Anti-Human DDX1 Polyclonal Antibody (HP260014) is a rabbit polyclonal antibody detecting DDX1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Chicken, Human, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human DDX1 (Gln72-Leu283).

靶标

DEAD box protein retinoblastoma, ATP-dependent RNA helicase DDX1, DEAD box protein 1, DBP-RB, DDX1

纯化方式

Purified by antigen affinity column.

Accession号

Q92499

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

ATP-dependent RNA helicase DDX1 is a ~82 kDa protein. Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation.

1. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-DDX1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: PC3 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: H1299 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: K562 cell lysate
Lane 9: Rat brain lysate
Lane 10: Mouse kidney lysate

参考文献

产品说明书

Anti-Human DDX1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human DDX1 Polyclonal Antibody (HP260014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human DDX1 Polyclonal Antibody (HP260014)

概述

图片

参考文献

产品说明书

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