Anti-Human GBP1 Polyclonal Antibody (HW466014)

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概述
图片
参考文献
产品说明书

概述

货号

HW466014

品牌

abinScience

描述

Anti-Human GBP1 Polyclonal Antibody (HW466014) is a rabbit polyclonal antibody detecting GBP1 in ELISA, IHC, WB. Suitable for Human and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human GBP1 (Met1-Ser592).

靶标

Guanine nucleotide-binding protein 1, HuGBP-1, GBP-1, Interferon-induced guanylate-binding protein 1, GBP1, GTP-binding protein 1, Guanylate-binding protein 1

纯化方式

Purified by antigen affinity column.

Accession号

P32455

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Guanylate-binding protein 1 (GBP1) is a ~67 kDa protein. Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. Hydrolyzes GTP to GMP in two consecutive cleavage reactions: GTP is first hydrolyzed to GDP and then to GMP in a processive manner. Following infection, recruited to the pathogen-containing vacuoles or vacuole-escaped bacteria and promotes both inflammasome assembly and autophagy. Acts as a positive regulator of inflammasome assembly by facilitating the detection of inflammasome ligands from pathogens. Involved in the lysis of pathogen-containing vacuoles, releasing pathogens into the cytosol.

1. Ghosh, A. et al. (2006) Nature 440, 101-4. PMID: 16511497

2. Nordmann, A. et al. (2012) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26, 1290-300. PMID: 22106366

3. Li, P. et al. (2017) Nature 551, 378-383. PMID: 29144452

4. Fisch, D. et al. (2019) The EMBO journal 38, e100926. PMID: 31268602

5. Kutsch, M. et al. (2020) The EMBO journal 39, e104926. PMID: 32510692

6. Santos, JC. et al. (2020) Nature communications 11, 3276. PMID: 32581219

7. Fisch, D. et al. (2023) Science (New York, N.Y.) 382, eadg2253. PMID: 37797010

8. Schwemmle, M. et al. (1994) The Journal of biological chemistry 269, 11299-305. PMID: 7512561

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-GBP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: MDA-MB-231 cell lysate
Lane 2: HUVEC cell lysate
Lane 3: Hela cell lysate
Lane 4: Jurkat cell lysate

参考文献

产品说明书

Anti-Human GBP1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human GBP1 Polyclonal Antibody (HW466014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human GBP1 Polyclonal Antibody (HW466014)

概述

图片

参考文献

产品说明书

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