Anti-Human YWHAE Polyclonal Antibody (HX044014)

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概述

货号

HX044014

品牌

abinScience

描述

Anti-Human YWHAE Polyclonal Antibody (HX044014) is a rabbit polyclonal antibody detecting YWHAE in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human YWHAE (Met1-Gln255).

靶标

14-3-3E, YWHAE, 14-3-3 protein epsilon

纯化方式

Purified by antigen affinity column.

Accession号

P62258

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

14-3-3 protein epsilon (YWHAE) is a ~29 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm. Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI.

1. Okamoto, Y. et al. (2011) The Journal of biological chemistry 286, 7171-81. PMID: 21189250

2. Zhang, ZD. et al. (2022) Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9, e2105391. PMID: 35343654

3. Wang, X. et al. (2003) Molecular and cellular biology 23, 6013-26. PMID: 12917326

4. Andrews, DDT. et al. (2023) Journal of virology 97, e0060423. PMID: 37555661

5. Liu, HM. et al. (2012) Cell host & microbe 11, 528-37. PMID: 22607805

6. Guo, M. et al. (2023) Cancer biology & therapy 24, 2246203. PMID: 37599448

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-YWHAE polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: A375 cell lysate
Lane 7: 293T cell lysate

参考文献

产品说明书

Anti-Human YWHAE Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human YWHAE Polyclonal Antibody (HX044014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human YWHAE Polyclonal Antibody (HX044014)

概述

图片

参考文献

产品说明书

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