Anti-Human ATP6V1B2 Polyclonal Antibody (HB120014)

价格:

规格：

50ug
100ug

数量：

一键复制产品信息

概述
图片
参考文献
产品说明书

HB120014

abinScience

种属反应性

Human, Mouse, Rat

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human ATP6V1B2 (Ser51-His511).

Endomembrane proton pump 58 kDa subunit, ATP6B2, V-type proton ATPase subunit B, brain isoform, HO57, VPP3, Vacuolar proton pump subunit B 2, ATP6V1B2, V-ATPase subunit B 2

Purified by antigen affinity column.

Accession号

P21281

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

V-type proton ATPase subunit B, brain isoform (ATP6V1B2) is a ~56 kDa protein. Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load.

1. Wang, L. et al. (2020) Molecular cell 80, 501-511.e3. PMID: 33065002

2. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091

For research use only.

Western blot

Western blot analysis was performed using anti-ATP6V1B2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Mouse kidney lysate
Lane 3: Mouse brain lysate
Lane 4: Rat kidney lysate
Lane 5: Rat brain lysate

产品说明书

Anti-Human ATP6V1B2 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATP6V1B2 Polyclonal Antibody (HB120014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATP6V1B2 Polyclonal Antibody (HB120014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价