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Anti-Human ATP6V1B2 Polyclonal Antibody (PTX20147)

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概述
货号PTX20147
品牌ProteoGenix
描述
Anti-Human ATP6V1B2 Polyclonal Antibody (PTX20147) is a rabbit polyclonal antibody detecting Endomembrane proton pump 58 kDa subunit, ATP6B2, V-type proton ATPase subunit B, brain isoform, HO57, VPP3, Vacuolar proton pump subunit B 2, ATP6V1B2, V-ATPase subunit B 2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human ATP6V1B2 (Ser51-His511).
靶标Endomembrane proton pump 58 kDa subunit, ATP6B2, V-type proton ATPase subunit B, brain isoform, HO57, VPP3, Vacuolar proton pump subunit B 2, ATP6V1B2, V-ATPase subunit B 2
纯化方式Purified by antigen affinity column.
Accession号P21281
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

V-type proton ATPase subunit B, brain isoform (ATP6V1B2) is a ~56 kDa protein. Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load.

1. Wang, L. et al. (2020) Molecular cell 80, 501-511.e3. PMID: 33065002
2. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091
NoteFor research use only.
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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Anti-Human ATP6V1B2 Polyclonal Antibody

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