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Anti-Human OS9 Polyclonal Antibody (HC357014)

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概述
货号HC357014
品牌abinScience
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human OS9 (Asn32-Gln251).
靶标Protein OS-9, OS9, Amplified in osteosarcoma 9
纯化方式Purified by antigen affinity column.
Accession号Q13438
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Protein OS-9 (OS9) is a ~75 kDa protein. Lectin component of the HRD1 complex, which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). Specifically recognizes and binds improperly folded glycoproteins as well as hyperglycosylated proteins, retain them in the ER, and transfers them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4 as well as hyperglycosylated HSP90B1.

1. Christianson, JC. et al. (2008) Nature cell biology 10, 272-82. PMID: 18264092
2. Bernasconi, R. et al. (2008) The Journal of biological chemistry 283, 16446-54. PMID: 18417469
3. Alcock, F. et al. (2009) Journal of molecular biology 385, 1032-42. PMID: 19084021
4. Hosokawa, N. et al. (2009) The Journal of biological chemistry 284, 17061-17068. PMID: 19346256
5. Satoh, T. et al. (2010) Molecular cell 40, 905-16. PMID: 21172656
6. Dersh, D. et al. (2014) Molecular biology of the cell 25, 2220-34. PMID: 24899641
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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