Anti-Human RFWD3 Polyclonal Antibody (HP698014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

HP698014

abinScience

种属反应性

Human

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human RFWD3 (Gly430-Glu774).

RING finger and WD repeat domain-containing protein 3, RNF201, E3 ubiquitin-protein ligase RFWD3, RING finger protein 201, RFWD3

Purified by antigen affinity column.

Accession号

Q6PCD5

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

E3 ubiquitin-protein ligase RFWD3 is a ~85 kDa protein. E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome.

1. Gong, Z. et al. (2011) The Journal of biological chemistry 286, 22308-13. PMID: 21504906

2. Liu, S. et al. (2011) The Journal of biological chemistry 286, 22314-22. PMID: 21558276

3. Elia, AE. et al. (2015) Molecular cell 60, 280-93. PMID: 26474068

4. Feeney, L. et al. (2017) Molecular cell 66, 610-621.e4. PMID: 28575657

5. Inano, S. et al. (2017) Molecular cell 66, 622-634.e8. PMID: 28575658

6. Gallina, I. et al. (2021) Molecular cell 81, 442-458.e9. PMID: 33321094

For research use only.

产品说明书

Anti-Human RFWD3 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human RFWD3 Polyclonal Antibody (HP698014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human RFWD3 Polyclonal Antibody (HP698014)

概述

图片

参考文献

产品说明书

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