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Anti-Human DDX3X Polyclonal Antibody (HT313014)

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概述
货号HT313014
品牌abinScience
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human DDX3X (Glu161-Ser410).
靶标DEAD box, X isoform, CAP-Rf, DDX3, HLP2, DEAD box protein 3, X-chromosomal, DDX3X, ATP-dependent RNA helicase DDX3X, Helicase-like protein 2, DBX
纯化方式Purified by antigen affinity column.
Accession号O00571
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

ATP-dependent RNA helicase DDX3X is a ~73 kDa protein. Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs. Binds RNA G-quadruplex (rG4s) structures, including those located in the 5'-UTR of NRAS mRNA. Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities (Probable).

1. Franca, R. et al. (2007) Proteins 67, 1128-37. PMID: 17357160
2. Garbelli, A. et al. (2011) PloS one 6, e19810. PMID: 21589879
3. Heaton, SM. et al. (2019) Cells 8. PMID: 31575075
4. Dolde, C. et al. (2018) Journal of cell science 131. PMID: 29222110
5. Herdy, B. et al. (2018) Nucleic acids research 46, 11592-11604. PMID: 30256975
6. Chao, CH. et al. (2006) Cancer research 66, 6579-88. PMID: 16818630
7. Botlagunta, M. et al. (2008) Oncogene 27, 3912-22. PMID: 18264132
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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