Anti-Human RIOK3 Polyclonal Antibody (HT159014)

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50ug
100ug

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概述
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参考文献
产品说明书

HT159014

abinScience

种属反应性

Human, Mouse

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human RIOK3 (Ser247-Glu519).

Serine/threonine-protein kinase RIO3, SUDD, sudD homolog, RIO kinase 3, RIOK3

Purified by antigen affinity column.

Accession号

O14730

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Serine/threonine-protein kinase RIO3 (RIOK3) is a ~59 kDa protein. Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Acts downstream of RNF10: specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner. The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling.

1. Coria, AR. et al. (2025) Molecular cell 85, 787-801.e8. PMID: 39947182

2. Huang, Z. et al. (2025) Molecular cell 85, 802-814.e12. PMID: 39947183

3. Ford, PW. et al. (2025) Cell reports 44, 115371. PMID: 40022732

4. Feng, J. et al. (2014) Journal of virology 88, 7987-97. PMID: 24807708

For research use only.

Western blot

Western blot analysis was performed using anti-RIOK3 polyclonal antibody at 1µg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: Mouse testis lysate
Lane 5: Rat testis lysate

产品说明书

Anti-Human RIOK3 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human RIOK3 Polyclonal Antibody (HT159014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human RIOK3 Polyclonal Antibody (HT159014)

概述

图片

参考文献

产品说明书

相关产品推荐

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