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Anti-MYL9 Polyclonal Antibody (HB901014)

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规格:
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  • 100ug
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  • 参考文献

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概述
货号HB901014
品牌abinScience
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human MYL9 (Arg5-Leu163).
靶标Myosin regulatory light chain MRLC1, 20 kDa myosin light chain, Myosin regulatory light chain 2, smooth muscle isoform, MYRL2, MYL9, MLC-2C, LC20, Myosin regulatory light chain 9, MLC2, MRLC1, Myosin RLC, Myosin regulatory light polypeptide 9
纯化方式Purified by antigen affinity column.
Accession号P24844
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Myosin regulatory light polypeptide 9 (MYL9) is a ~19 kDa protein. Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation.

1. Iwasaki, T. et al. (2001) Cell structure and function 26, 677-83. PMID: 11942626
2. Kumar, CC. et al. (1989) Biochemistry 28, 4027-35. PMID: 2526655
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-MYL9 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: C2C12 cell lysate
    Lane 2: Caco2 cell lysate
    Lane 3: Daudi cell lysate
    Lane 4: PC12 cell lysate
    Lane 5: mouse Lung
    Lane 6: Mouse colon lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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