Anti-LEF1 Polyclonal Antibody (HV355014)

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50ug
100ug

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HV355014

abinScience

种属反应性

Human, Mouse, Rat

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human LEF1 (Glu45-His260).

Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, LEF-1, TCF1-alpha, LEF1

Purified by antigen affinity column.

Accession号

Q9UJU2

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Lymphoid enhancer-binding factor 1 (LEF1) is a ~44 kDa protein. Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1.

1. Waterman, ML. et al. (1991) Genes & development 5, 656-69. PMID: 2010090

2. Brantjes, H. et al. (2001) Nucleic acids research 29, 1410-9. PMID: 11266540

3. Jesse, S. et al. (2010) International journal of cancer 126, 1109-20. PMID: 19653274

For research use only.

Western blot

Western blot analysis was performed using anti-LEF1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Jurkat cell lysate
Lane 2: HepG2 cell lysate
Lane 3: BxPC3 cell lysate
Lane 4: K562 cell lysate
Lane 5: Mouse thymus lysate

产品说明书

Anti-LEF1 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

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实验相关试剂

Anti-LEF1 Polyclonal Antibody (HV355014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-LEF1 Polyclonal Antibody (HV355014)

概述

图片

参考文献

产品说明书

相关产品推荐

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