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Anti-LEF1 Polyclonal Antibody (ARO-A11729)

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概述
货号ARO-A11729
品牌ProteoGenix
描述
Anti-LEF1 Polyclonal Antibody (ARO-A11729) is a rabbit polyclonal antibody detecting Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, LEF-1, TCF1-alpha, LEF1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human LEF1 (Glu45-His260).
靶标Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, LEF-1, TCF1-alpha, LEF1
纯化方式Purified by antigen affinity column.
Accession号Q9UJU2
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Lymphoid enhancer-binding factor 1 (LEF1) is a ~44 kDa protein. Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1.

1. Waterman, ML. et al. (1991) Genes & development 5, 656-69. PMID: 2010090
2. Brantjes, H. et al. (2001) Nucleic acids research 29, 1410-9. PMID: 11266540
3. Jesse, S. et al. (2010) International journal of cancer 126, 1109-20. PMID: 19653274
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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Anti-LEF1 Polyclonal Antibody

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