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Anti-AP2M1 Polyclonal Antibody (HV707014)

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规格:
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  • 100ug
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概述
货号HV707014
品牌abinScience
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human AP2M1 (Arg170-Cys435).
靶标Adaptor-related protein complex 2 subunit mu, AP-2 mu chain, CLAPM1, HA2 50 kDa subunit, Plasma membrane adaptor AP-2 50 kDa protein, Adaptin-mu2, AP2M1, KIAA0109, Clathrin coat assembly protein AP50, Adaptor protein complex AP-2 subunit mu, Clathrin assembly protein complex 2 mu medium chain, AP-2 complex subunit mu, Clathrin coat-associated protein AP50
纯化方式Purified by antigen affinity column.
Accession号Q96CW1
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

AP-2 complex subunit mu (AP2M1) is a ~49 kDa protein. Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.

1. Fraile-Ramos, A. et al. (2003) Traffic (Copenhagen, Denmark) 4, 243-53. PMID: 12694563
2. Motley, A. et al. (2003) The Journal of cell biology 162, 909-18. PMID: 12952941
3. Nakatsu, F. et al. (2003) Cell structure and function 28, 419-29. PMID: 14745134
4. Huang, F. et al. (2004) The Journal of biological chemistry 279, 16657-61. PMID: 14985334
5. Owen, DJ. et al. (2004) Annual review of cell and developmental biology 20, 153-91. PMID: 15473838
6. Helbig, I. et al. (2019) American journal of human genetics 104, 1060-1072. PMID: 31104773
9. Marcello, E. et al. (2013) The Journal of clinical investigation 123, 2523-38. PMID: 23676497
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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