ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
32.5 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Adaptor-related protein complex 2 subunit mu, AP-2 mu chain, CLAPM1, HA2 50 kDa subunit, Plasma membrane adaptor AP-2 50 kDa protein, Adaptin-mu2, AP2M1, KIAA0109, Clathrin coat assembly protein AP50, Adaptor protein complex AP-2 subunit mu, Clathrin assembly protein complex 2 mu medium chain, AP-2 complex subunit mu, Clathrin coat-associated protein AP50
背景
AP-2 complex subunit mu (AP2M1) is a ~49 kDa protein. Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.
1. Fraile-Ramos, A. et al. (2003) Traffic (Copenhagen, Denmark) 4, 243-53. PMID: 12694563
2. Motley, A. et al. (2003) The Journal of cell biology 162, 909-18. PMID: 12952941
3. Nakatsu, F. et al. (2003) Cell structure and function 28, 419-29. PMID: 14745134
4. Huang, F. et al. (2004) The Journal of biological chemistry 279, 16657-61. PMID: 14985334
5. Owen, DJ. et al. (2004) Annual review of cell and developmental biology 20, 153-91. PMID: 15473838
6. Helbig, I. et al. (2019) American journal of human genetics 104, 1060-1072. PMID: 31104773
9. Marcello, E. et al. (2013) The Journal of clinical investigation 123, 2523-38. PMID: 23676497
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human AP2M1 Protein, N-His
