Anti-ALOX15B Polyclonal Antibody (HT104014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

HT104014

abinScience

种属反应性

Human

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human ALOX15B (Ala2-Gly121).

15-lipoxygenase 2, 15-LOX-B, 15-LOX-2, Linoleate 13-lipoxygenase 15-LOb, Arachidonate 15-lipoxygenase B, Polyunsaturated fatty acid lipoxygenase ALOX15B, ALOX15B, Arachidonate 15-lipoxygenase type II

Purified by antigen affinity column.

Accession号

O15296

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Polyunsaturated fatty acid lipoxygenase ALOX15B is a ~75 kDa protein. Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist. Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates.

1. Kilty, I. et al. (1999) European journal of biochemistry 266, 83-93. PMID: 10542053

2. Jisaka, M. et al. (2000) The Journal of biological chemistry 275, 1287-93. PMID: 10625675

3. Bhatia, B. et al. (2003) The Journal of biological chemistry 278, 25091-100. PMID: 12704195

4. Prusakiewicz, JJ. et al. (2007) Archives of biochemistry and biophysics 464, 260-8. PMID: 17493578

5. Turman, MV. et al. (2008) Biochemistry 47, 3917-25. PMID: 18311922

6. Horn, T. et al. (2013) Redox biology 1, 566-77. PMID: 24282679

7. Kobe, MJ. et al. (2014) The Journal of biological chemistry 289, 8562-9. PMID: 24497644

8. Perry, SC. et al. (2020) Journal of lipid research 61, 1087-1103. PMID: 32404334

9. Brash, AR. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 6148-52. PMID: 9177185

For research use only.

产品说明书

Anti-ALOX15B Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ALOX15B Polyclonal Antibody (HT104014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ALOX15B Polyclonal Antibody (HT104014)

概述

图片

参考文献

产品说明书

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