Anti-Human GATM Polyclonal Antibody (HW668014)

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50ug
100ug

数量：

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概述
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产品说明书

概述

货号

HW668014

品牌

abinScience

描述

Anti-Human GATM Polyclonal Antibody (HW668014) is a rabbit polyclonal antibody detecting GATM in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human GATM (Glu76-Asp423).

靶标

Glycine amidinotransferase, mitochondrial, L-arginine:glycine amidinotransferase, GATM, Transamidinase, AGAT

纯化方式

Purified by antigen affinity column.

Accession号

P50440

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Glycine amidinotransferase, mitochondrial (GATM) is a ~48 kDa protein. Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives. Catalyzes the rate-limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable).

1. Cullen, ME. et al. (2006) Circulation 114, I16-20. PMID: 16820567

2. DesRoches, CL. et al. (2016) Human mutation 37, 926-32. PMID: 27233232

3. Sinn, M. et al. (2022) Scientific reports 12, 22088. PMID: 36543883

4. Gross, MD. et al. (1986) Archives of biochemistry and biophysics 251, 747-55. PMID: 3800397

5. Fritsche, E. et al. (1997) European journal of biochemistry 247, 483-90. PMID: 9266688

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-GATM1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse kidney lysate
Lane 2: Mouse pancears lysate
Lane 3: Rat kidney lysate
Lane 4: Rat pancears lysat

参考文献

产品说明书

Anti-Human GATM Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human GATM Polyclonal Antibody (HW668014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human GATM Polyclonal Antibody (HW668014)

概述

图片

参考文献

产品说明书

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