Anti-Mouse SPHK2 Polyclonal Antibody (MP741014)

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50ug
100ug

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概述
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参考文献
产品说明书

概述

货号

MP741014

品牌

abinScience

描述

Anti-Mouse SPHK2 Polyclonal Antibody (MP741014) is a rabbit polyclonal antibody detecting SPHK2 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Mouse SPHK2 (Ile15-Gly276).

靶标

SK2, Sphingosine kinase 2, SPHK2, SK 2, SPK 2

纯化方式

Purified by antigen affinity column.

Accession号

Q9JIA7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Sphingosine kinase 2 (SPHK2) is a ~65 kDa protein. Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. In contrast to prosurvival SPHK1, has a positive effect on intracellular ceramide levels, inhibits cells growth and enhances apoptosis. In mitochondria, is important for cytochrome-c oxidase assembly and mitochondrial respiration.

1. Maceyka, M. et al. (2005) The Journal of biological chemistry 280, 37118-29. PMID: 16118219

2. Olivera, A. et al. (2007) Immunity 26, 287-97. PMID: 17346996

3. Hisano, Y. et al. (2011) The Journal of biological chemistry 286, 1758-66. PMID: 21084291

4. Liu, H. et al. (2000) The Journal of biological chemistry 275, 19513-20. PMID: 10751414

5. Strub, GM. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 600-12. PMID: 20959514

6. Sivasubramanian, M. et al. (2015) Neuroscience 290, 636-48. PMID: 25637806

7. Ravichandran, S. et al. (2019) Biochimica et biophysica acta. Molecular basis of disease 1865, 570-576. PMID: 30593892

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-SPHK2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HCT116 cell lysate
Lane 2: Raji cell lysate
Lane 3: LO2 cell lysate

参考文献

产品说明书

Anti-Mouse SPHK2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse SPHK2 Polyclonal Antibody (MP741014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse SPHK2 Polyclonal Antibody (MP741014)

概述

图片

参考文献

产品说明书

相关产品推荐

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