Anti-BECN1 Polyclonal Antibody (HC377024)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HC377024

品牌

abinScience

描述

Anti-BECN1 Polyclonal Antibody (HC377024) is a rabbit polyclonal antibody detecting BECN1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human BECN1 (Thr141-Lys450).

靶标

BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, GT197

纯化方式

Purified by antigen affinity column.

Accession号

Q14457

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Beclin-1 (BECN1) is a ~51 kDa protein. Plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis.

1. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

2. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617

3. Margariti, A. et al. (2013) The Journal of biological chemistry 288, 859-72. PMID: 23184933

4. Xia, P. et al. (2013) The EMBO journal 32, 2685-96. PMID: 23974797

5. Xu, C. et al. (2014) Autophagy 10, 2239-50. PMID: 25484083

6. Ashkenazi, A. et al. (2017) Nature 545, 108-111. PMID: 28445460

7. Wyatt, S. et al. (2023) Biochemistry 62, 2934-2951. PMID: 37776275

8. Sagona, AP. et al. (2010) Nature cell biology 12, 362-71. PMID: 20208530

9. Thoresen, SB. et al. (2010) Experimental cell research 316, 3368-78. PMID: 20643123

10. Liu, K. et al. (2016) The Journal of cell biology 212, 181-98. PMID: 26783301

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-Beclin-1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Mouse testis lysate
Lane 3: Mouse brain lysate
Lane 4: Mouse lung lysate
Lane 5: Rat testis lysate
Lane 6: Rat brain lysate
Lane 7: Rat lung lysate

参考文献

产品说明书

Anti-BECN1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-BECN1 Polyclonal Antibody (HC377024)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-BECN1 Polyclonal Antibody (HC377024)

概述

图片

参考文献

产品说明书

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