Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

VK686014

品牌

abinScience

描述

Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014) is a rabbit polyclonal antibody detecting EBNA1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Epstein-Barr virus (HHV-4) (Human herpesvirus 4)

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant EBV/HHV4 EBNA1/BKRF1 (His468-Pro607).

靶标

Epstein-Barr nuclear antigen 1, EBNA-1, EBV nuclear antigen 1, EBNA1, BKRF1

纯化方式

Purified by antigen affinity column.

Accession号

P03211

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Epstein-Barr nuclear antigen 1 (EBNA1/BKRF1) is a ~56 kDa protein. Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection. EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency. EBNA1 binding to DS recruits the host origin recognition complex (ORC). Governs the faithful mitotic segregation of the viral episomes by binding both the FR (family of repeats) element within oriP and the host mitotic chromosomes. Forms a cell cycle-dependent tyrosine-dependent DNA cross-link and single-strand cleavage at oriP required for terminating replication and maintaining viral episomes.

1. Sears, J. et al. (2004) Journal of virology 78, 11487-505. PMID: 15479791

2. Rawlins, DR. et al. (1985) Cell 42, 859-68. PMID: 2996781

3. Hodin, TL. et al. (2013) Journal of virology 87, 13020-8. PMID: 24067969

4. Malecka, KA. et al. (2019) Journal of virology 93. PMID: 31142669

5. Summers, H. et al. (1996) Journal of virology 70, 1228-31. PMID: 8551585

6. Ritzi, M. et al. (2003) Journal of cell science 116, 3971-84. PMID: 12953058

7. Hung, SC. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1865-70. PMID: 11172042

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-EBNA1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EBV/HHV4 EBNA1/BKRF1 (Catalog No: VK686012)

参考文献

产品说明书

Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014)

概述

图片

参考文献

产品说明书

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