ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Epstein-Barr virus (HHV-4) (Human herpesvirus 4)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
17.52 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Epstein-Barr nuclear antigen 1 (EBNA1/BKRF1) is a ~56 kDa protein. Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection. EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency. EBNA1 binding to DS recruits the host origin recognition complex (ORC). Governs the faithful mitotic segregation of the viral episomes by binding both the FR (family of repeats) element within oriP and the host mitotic chromosomes. Forms a cell cycle-dependent tyrosine-dependent DNA cross-link and single-strand cleavage at oriP required for terminating replication and maintaining viral episomes.
1. Sears, J. et al. (2004) Journal of virology 78, 11487-505. PMID: 15479791
2. Rawlins, DR. et al. (1985) Cell 42, 859-68. PMID: 2996781
3. Hodin, TL. et al. (2013) Journal of virology 87, 13020-8. PMID: 24067969
4. Malecka, KA. et al. (2019) Journal of virology 93. PMID: 31142669
5. Summers, H. et al. (1996) Journal of virology 70, 1228-31. PMID: 8551585
6. Ritzi, M. et al. (2003) Journal of cell science 116, 3971-84. PMID: 12953058
7. Hung, SC. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1865-70. PMID: 11172042
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for EBV/HHV4 EBNA1/BKRF1 Recombinant Protein (N-His)
