Anti-HSPA5 Polyclonal Antibody (HY489024) is a rabbit polyclonal antibody detecting HSPA5 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human HSPA5 (Leu351-Leu654).
靶标
Heat shock protein 70 family protein 5, 78 kDa glucose-regulated protein, HSPA5, Binding-immunoglobulin protein, GRP-78, HSP70 family protein 5, Heat shock protein family A member 5, Endoplasmic reticulum chaperone BiP, GRP78, BiP, Immunoglobulin heavy chain-binding protein
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Endoplasmic reticulum chaperone BiP (HSPA5/GRP78) is a ~72 kDa protein. Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Also binds and inactivates EIF2AK3/PERK in unstressed cells.
1. Dana, RC. et al. (1990) Endocrinology 126, 672-4. PMID: 2294010
5. Ma, K. et al. (2002) The Journal of biological chemistry 277, 18728-35. PMID: 11907036
6. Ng, DT. et al. (1992) Molecular biology of the cell 3, 143-55. PMID: 1550958
7. Oikawa, D. et al. (2009) Experimental cell research 315, 2496-504. PMID: 19538957
8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529
9. Kang, JM. et al. (2015) Cancer research 75, 3087-97. PMID: 26045166
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-HSPA5 polyclonal antibody at 1ug/mL on various samples. Lane 1: HepG2 cell lysate Lane 2: NIH3T3 cell lysate Lane 3: 4T1 cell lysate Lane 4: MCF-7 cell lysate Lane 5: Jurkat cell lysate Lane 6: PC-12 cell lysate Lane 7: Hela cell lysate Lane 8: A549 cell lysate Lane 9: Mouse brain lysate Lane 10: Mouse liver lysate Lane11: Rat brain lysate Lane12: Rat liver lysate
