Anti-Human GRP78/HSPA5 (PAT-SM6) (HY489016) is a research-grade recombinant antibody targeting Heat shock protein 70 family protein 5. Produced in mammalian cells with native-like glycosylation.
Highlights
●Research Grade — For PK/PD studies, assay development, and ADA research.
ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主
Human
同种型
IgG
克隆号
PAT-SM6
表达系统
Mammalian cells
克隆类型
Monoclonal
靶标
Heat shock protein 70 family protein 5, 78 kDa glucose-regulated protein, HSPA5, Binding-immunoglobulin protein, GRP-78, HSP70 family protein 5, Heat shock protein family A member 5, Endoplasmic reticulum chaperone BiP, GRP78, BiP, Immunoglobulin heavy chain-binding protein
内毒素水平
< 10 EU/mg
纯度
>95% purity as determined by SDS-PAGE.
纯化方式
Protein A/G purified from cell culture supernatant.
Accession号
P11021
状态
Liquid
保存溶液
0.01M PBS pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
PAT-SM6, PATSM6, PATSM6
背景
Endoplasmic reticulum chaperone BiP (HSPA5/GRP78) is a ~72 kDa protein. Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Also binds and inactivates EIF2AK3/PERK in unstressed cells.
1. Dana, RC. et al. (1990) Endocrinology 126, 672-4. PMID: 2294010
