Anti-Human POLR3D Polyclonal Antibody (HY534014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HY534014

品牌

abinScience

描述

Anti-Human POLR3D Polyclonal Antibody (HY534014) is a rabbit polyclonal antibody detecting POLR3D in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human POLR3D (Val249-Arg398).

靶标

DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III subunit C4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47 kDa subunit, RPC53 homolog, POLR3D, BN51, BN51T

纯化方式

Purified by antigen affinity column.

Accession号

P05423

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA-directed RNA polymerase III subunit RPC4 (POLR3D) is a ~44 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Assembles with POLR3E/RPC5 forming a subcomplex that binds the Pol III core. Enables recruitment of Pol III at transcription initiation site and drives transcription initiation from both type 2 and type 3 DNA promoters. Required for efficient transcription termination and reinitiation.

1. Hu, P. et al. (2002) Molecular and cellular biology 22, 8044-55. PMID: 12391170

2. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673

3. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764

4. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218

5. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192

6. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254

7. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human POLR3D Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLR3D Polyclonal Antibody (HY534014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLR3D Polyclonal Antibody (HY534014)

概述

图片

参考文献

产品说明书

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