Anti-ORF8/ns8 Polyclonal Antibody (VK460014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

VK460014

品牌

abinScience

描述

Anti-ORF8/ns8 Polyclonal Antibody (VK460014) is a rabbit polyclonal antibody detecting ORF8 protein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant SARS-CoV-2 ORF8/ns8 (Phe16-Ile121).

靶标

ORF8 protein, ORF8, Non-structural protein 8, ns8

纯化方式

Purified by antigen affinity column.

Accession号

P0DTC8

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

ORF8 protein (ns8) is a ~13 kDa protein. Plays a role in modulating the host immune response. May act as a secreted virokine by mimicking interleukin-17A (IL17A), and thereby binding to the IL17RA receptor, leading to activation of the IL17 pathway and increased secretion of pro-inflammatory factors. Contributes to the cytokine storm during SARS-CoV-2 infection when secreted by unconventional pathway. May act by down-regulating major histocompability complex class I (MHC-I) at cell surface. May inhibit expression of some members of the IFN-stimulated gene (ISG) family including hosts IGF2BP1/ZBP1, MX1 and MX2, and DHX58.

1. Chan, JF. et al. (2020) Lancet (London, England) 395, 514-523. PMID: 31986261

2. Wu, X. et al. (2022) mBio 13, e0040222. PMID: 35343786

3. Lin, X. et al. (2023) PLoS pathogens 19, e1011128. PMID: 36689483

4. Lin, X. et al. (2021) iScience 24, 102293. PMID: 33723527

5. Zhang, Y. et al. (2021) Proceedings of the National Academy of Sciences of the United States of America 118. PMID: 34021074

6. Matsuoka, K. et al. (2022) The Journal of biological chemistry 298, 101724. PMID: 35157849

7. Geng, H. et al. (2021) Frontiers in immunology 12, 679482. PMID: 34177923

Note

For research use only.

图片

参考文献

产品说明书

Anti-ORF8/ns8 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ORF8/ns8 Polyclonal Antibody (VK460014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ORF8/ns8 Polyclonal Antibody (VK460014)

概述

图片

参考文献

产品说明书

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