Anti-Human SOD1/Cu-Zn SOD Recombinant Nanobody (SAA1203) (HF876013)

价格: --

规格：

50ug
100ug
1mg

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HF876013

品牌

abinScience

描述

Anti-Human SOD1 Nanobody (SAA1203) [SAA1203] (HF876013) is a alpaca monoclonal antibody detecting SOD1 in ELISA. Suitable for Human.

种属反应性

Human

应用

ELISA, WB

宿主

Alpaca

克隆类型

Monoclonal

同种型

VHH-8His-Cys-tag

克隆号

SAA1203

经测试应用

WB, IF

靶标

Superoxide dismutase [Cu-Zn], SOD1, hSod1, Superoxide dismutase 1

内毒素水平

< 10 EU/mg as determined by LAL method.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

P00441

状态

Liquid

保存溶液

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Superoxide dismutase [Cu-Zn] (SOD1) is a ~15 kDa protein. Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides.

1. Lin, ZF. et al. (2013) Biochemical and biophysical research communications 441, 191-5. PMID: 24140062

2. Switzer, CH. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2205044120. PMID: 36630448

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human SOD1 Nanobody (SAA1203)
Bioactivity

Detects recombinant SOD1 (Catalog No: HF876012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-SOD1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human SOD1 (Catalog No: HF876012)
Immunofluorescence

Immunofluorescence staining of PC12 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cytoplasm .

参考文献

产品说明书

Anti-Human SOD1/Cu-Zn SOD Recombinant Nanobody (SAA1203).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SOD1/Cu-Zn SOD Recombinant Nanobody (SAA1203) (HF876013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SOD1/Cu-Zn SOD Recombinant Nanobody (SAA1203) (HF876013)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价