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Anti-SOD1 Polyclonal Antibody (HF876014)

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规格:
  • 50ug
  • 100ug
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  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HF876014
品牌abinScience
描述
Anti-SOD1 Polyclonal Antibody (HF876014) is a rabbit polyclonal antibody detecting SOD1 in ELISA, IHC, WB. Suitable for Human, Callithrix jacchus, Camelus dromedarius, Cavia porcellus, and Hylobates lar.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human SOD1 (Met1-Gln154).
靶标Superoxide dismutase [Cu-Zn], SOD1, hSod1, Superoxide dismutase 1
纯化方式Purified by antigen affinity column.
Accession号P00441
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Superoxide dismutase [Cu-Zn] (SOD1) is a ~15 kDa protein. Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides.

1. Lin, ZF. et al. (2013) Biochemical and biophysical research communications 441, 191-5. PMID: 24140062
2. Switzer, CH. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2205044120. PMID: 36630448
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-SOD1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: K562 cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: MCF-7 cell lysate
    Lane 7: Mouse brain lysate
    Lane 8: Mouse liver lysate
    Lane 9: Rat brain lysate
    Lane 10: Rat liver lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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