Anti-Human Integrin beta 1/CD29 Recombinant Nanobody (SAA1306) (HY248013)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HY248013

品牌

abinScience

描述

Anti-Human CD29/ITGB1 Nanobody (SAA1306) [SAA1306] (HY248013) is a alpaca monoclonal antibody detecting ITGB1 in ELISA. Suitable for Human.

种属反应性

Human

应用

ELISA

宿主

Alpaca

克隆类型

Monoclonal

同种型

VHH-8His-Cys-tag

克隆号

SAA1306

经测试应用

靶标

MSK12, VLA-4 subunit beta, CD29, Integrin beta-1, ITGB1, FNRB, MDF2, GPIIA, Glycoprotein IIa, Fibronectin receptor subunit beta

内毒素水平

< 10 EU/mg as determined by LAL method.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

P05556

状态

Liquid

保存溶液

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Integrin beta-1 (CD29/ITGB1) is a ~88 kDa protein. Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen.

1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415

2. Fujita, M. et al. (2014) PloS one 9, e96372. PMID: 24789099

3. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536

4. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877

5. Bax, DV. et al. (2003) The Journal of biological chemistry 278, 34605-16. PMID: 12807887

6. Jovanovic, J. et al. (2007) The Journal of biological chemistry 282, 6743-51. PMID: 17158881

7. Schumacher, S. et al. (2021) Science advances 7. PMID: 33962943

8. Takada, YK. et al. (2017) The Journal of biological chemistry 292, 20067-20075. PMID: 29030430

9. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973

10. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human CD29/ITGB1 Nanobody (SAA1306).
Bioactivity

Detects recombinant CD29 (Catalog No: HY248011) in indirect ELISA.
Immunofluorescence

Immunofluorescence staining of Hepg2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane.

参考文献

产品说明书

Anti-Human Integrin beta 1/CD29 Recombinant Nanobody (SAA1306).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human Integrin beta 1/CD29 Recombinant Nanobody (SAA1306) (HY248013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human Integrin beta 1/CD29 Recombinant Nanobody (SAA1306) (HY248013)

概述

图片

参考文献

产品说明书

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