Anti-Human ASC/TMS1/PYCARD Recombinant Nanobody (SAA1187) (HV826013)

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50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HV826013

品牌

abinScience

描述

Anti-Human ASC/TMS1/PYCARD Nanobody (SAA1187) [SAA1187] (HV826013) is a alpaca monoclonal antibody detecting PYCARD in ELISA, IF, IP. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IF, IP

宿主

Alpaca

同种型

VHH-8His-Cys-tag

克隆号

SAA1187

克隆类型

Monoclonal

靶标

TMS1, CARD5, Caspase recruitment domain-containing protein 5, Apoptosis-associated speck-like protein containing a CARD, PYD and CARD domain-containing protein, ASC, PYCARD, hASC, Target of methylation-induced silencing 1

内毒素水平

Please contact with the lab for this information.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column

Accession号

Q9ULZ3

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human ASC/TMS1/PYCARD Nanobody (SAA1187)
Immunofluorescence

MCF7 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with His-tagged ASC protein (HV826013) at a concentration of 20 µg/mL overnight at 4°C. After washing, the cells were incubated with an anti-His primary antibody for 1 hour at room temperature, followed by incubation with Goat Anti-Mouse IgG (Alexa Fluor 488) for 50 minutes at room temperature. The localization of IDE was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

参考文献

产品说明书

Anti-Human ASC/TMS1/PYCARD Recombinant Nanobody (SAA1187).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ASC/TMS1/PYCARD Recombinant Nanobody (SAA1187) (HV826013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ASC/TMS1/PYCARD Recombinant Nanobody (SAA1187) (HV826013)

概述

图片

参考文献

产品说明书

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