Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0993) (JN085013)

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50ug
100ug
1mg

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概述
图片
参考文献
产品说明书

概述

货号

JN085013

品牌

abinScience

描述

Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993) [SAA0993] (JN085013) is a alpaca monoclonal antibody detecting Clostridium difficile Toxin in ELISA.

种属反应性

Clostridioides difficile

应用

ELISA, WB

宿主

Alpaca

同种型

VHH-8His-Cys-tag

克隆号

SAA0993

克隆类型

Monoclonal

经测试应用

靶标

Toxin A, 3.4.22.-, Glucosyltransferase TcdA, 2.4.1.-, tcdA, toxA, Clostridium difficile Toxin

内毒素水平

Please contact with the lab for this information.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

P16154

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Toxin A (tcdA) is a ~308 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdA and TcdB constitute the main toxins that mediate the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdB, TcdA is less virulent and less important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdA) into the host cytosol. Targets colonic epithelia by binding to some receptor, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

3. Greco, A. et al. (2006) Nature structural & molecular biology 13, 460-1. PMID: 16622409

4. Tucker, KD. et al. (1991) Infection and immunity 59, 73-8. PMID: 1670930

5. Tao, L. et al. (2019) Nature microbiology 4, 1760-1769. PMID: 31160825

6. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

8. Pruitt, RN. et al. (2009) The Journal of biological chemistry 284, 21934-21940. PMID: 19553670

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993)
Bioactivity

Detects Clostridioides difficile Toxin A (Catalog No: JN085012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-Toxin A monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridioides difficil Toxin A (Catalog No: JN085012)

参考文献

产品说明书

Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0993).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0993) (JN085013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0993) (JN085013)

概述

图片

参考文献

产品说明书

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