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Anti-Mouse VEGF Recombinant Antibody (G6) (MB941013)

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  • 1mg
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概述
货号MB941013
品牌abinScience
种属反应性Human, Mouse, Rat, Rabbit
应用Blocking, ELISA, FCM
同种型IgG2a, kappa
克隆号G6
经测试应用WB, FCM
靶标Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号Q00731
RRIDAnti-Mouse VEGFA Antibody (G6) (abinScience Cat# MB941013, RRID:AB_3727197)
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Vascular endothelial growth factor A, long form (VEGFA) is a ~43 kDa protein. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA is the therapeutic target of bevacizumab (Avastin).

1. Katsman, M. et al. (2022) Cells 11. PMID: 35455969
NoteFor research use only.
Isotype ControlMouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
图片
  • Bioactivity

    Detects Mouse VEGFA in indirect ELISAs.

  • SDS-PAGE

    SDS-PAGE for Anti-Mouse VEGFA Antibody (G6)

  • Flow CytoMetry

    Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: MB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011)
    Lane 2: negative control

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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