| 货号 | RB941013 | ||||
|---|---|---|---|---|---|
| 品牌 | abinScience | ||||
| 种属反应性 | Human, Mouse, Rat, Rabbit | ||||
| 应用 | Blocking, ELISA | ||||
| 宿主 | Mouse | ||||
| 同种型 | IgG2a, kappa | ||||
| 克隆号 | B20-4 | ||||
| 克隆类型 | Monoclonal | ||||
| 靶标 | Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A | ||||
| 内毒素水平 | Please contact with the lab for this information. | ||||
| 纯度 | >95% as determined by SDS-PAGE. | ||||
| 纯化方式 | Protein A/G purified from cell culture supernatant. | ||||
| Accession号 | P16612 | ||||
| 状态 | Liquid | ||||
| 保存溶液 | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| 产品使用信息 |
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Note | For research use only. |
Detects Rat VEGFA in indirect ELISAs.
SDS-PAGE for Anti-Rat VEGFA Antibody (B20-4).
Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011)
Lane 2: negative control
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