Anti-Human Bcl2 Recombinant Antibody (SAA1989) (HY131023)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HY131023

abinScience

种属反应性

Human

ELISA, FCM

VHH-mFc

SAA1989

Apoptosis regulator Bcl-2, BCL2

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P10415

Anti-Human Bcl2 Antibody (SAA1989) (abinScience Cat# HY131023, RRID:AB_3727774)
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Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Apoptosis regulator Bcl-2 (BCL2) is a ~26 kDa protein. Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function.

1. Eguchi, Y. et al. (1992) Nucleic acids research 20, 4187-92. PMID: 1508712

2. Yin, XM. et al. (1994) Nature 369, 321-3. PMID: 8183370

3. Ruvolo, PP. et al. (2001) Leukemia 15, 515-22. PMID: 11368354

4. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

5. Chen, D. et al. (2010) The Journal of biological chemistry 285, 38214-23. PMID: 20889974

6. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617

7. Bruey, JM. et al. (2007) Cell 129, 45-56. PMID: 17418785

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Flow CytoMetry

Flow-cytometry using PE anti-human BCL2 antibody. A549 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human BCL2 monoclonal antibody (Catalog: HY131023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human BCL2 antibody. A549 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human BCL2 monoclonal antibody (Catalog: HY131023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human Bcl2 Recombinant Antibody (SAA1989).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

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实验相关试剂

Anti-Human Bcl2 Recombinant Antibody (SAA1989) (HY131023)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human Bcl2 Recombinant Antibody (SAA1989) (HY131023)

概述

图片

参考文献

产品说明书

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