Anti-Human IL-6 Recombinant Antibody (SAA2009) (HY328043)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HY328043

abinScience

种属反应性

Human

ELISA, FCM, WB

IgG4, kappa

SAA2009

经测试应用

WB, FCM

B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, Interleukin-6

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P05231

Anti-Human IL6 Antibody (SAA2009) (abinScience Cat# HY328043, RRID:AB_3727921)
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Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Interleukin-6 (IL6) is a ~23 kDa protein. Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). IL6 is the therapeutic target of tocilizumab (Actemra).

For research use only.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity

SEC-HPLC detection for Anti-Human IL6 Antibody (ABS1221).
Flow Cytometry

Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328043 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human IL6 Antibody (SAA2009).
Western blot

Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL6 (Catalog No: HY328021), 50ng
Lane 2: recombinant human IL6 (Catalog No: HY328021), 30ng
Lane 3: recombinant human IL6 (Catalog No: HY328021), 20ng
Lane 4: recombinant human IL6 (Catalog No: HY328021), 10ng

产品说明书

Anti-Human IL-6 Recombinant Antibody (SAA2009).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IL-6 Recombinant Antibody (SAA2009) (HY328043)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IL-6 Recombinant Antibody (SAA2009) (HY328043)

概述

图片

参考文献

产品说明书

相关产品推荐

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