Anti-VDAC1 Recombinant Antibody (n152b/11) (HB031013)

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50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HB031013

品牌

abinScience

描述

Anti-VDAC1 Recombinant Antibody (N152B/11) (HB031013) is a mouse monoclonal antibody detecting VDAC1 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

应用

IHC, WB

宿主

Mouse

克隆类型

Monoclonal

同种型

IgG2a, kappa

克隆号

N152B/11

靶标

Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, hVDAC1, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC1, VDAC-1, VDAC

内毒素水平

< 10 EU/mg as determined by LAL method.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Protein A/G purified from cell culture supernatant.

RRID

Anti-VDAC1 Antibody (n152b/11) (abinScience Cat# HB031013, RRID:AB_3728157)
_x005F_x000D_

状态

Liquid

保存溶液

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

背景

Non-selective voltage-gated ion channel VDAC1 is a ~30 kDa protein. Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol.

1. Stadtmüller, U. et al. (1999) Biological chemistry 380, 1461-6. PMID: 10661876

2. Thinnes, FP. et al. (2001) Pflugers Archiv : European journal of physiology 443 Suppl 1, S111-6. PMID: 11845315

3. Hiller, S. et al. (2008) Science (New York, N.Y.) 321, 1206-10. PMID: 18755977

4. Marginedas-Freixa, I. et al. (2018) Scientific reports 8, 11384. PMID: 30061676

5. Blachly-Dyson, E. et al. (1993) The Journal of biological chemistry 268, 1835-41. PMID: 8420959

6. Dadsena, S. et al. (2019) Nature communications 10, 1832. PMID: 31015432

7. Ham, SJ. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 4281-4291. PMID: 32047033

10. Chen, Y. et al. (2010) Biochemical and biophysical research communications 394, 798-803. PMID: 20230784

Note

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

图片

Bioactivity

Detects Recombinant Human VDAC1 Protein, N-His (Cat No.: HB031012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-human VDAC1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human VDAC1 (Catalog No: HB031012)
Lane 2: negative control

参考文献

产品说明书

Anti-VDAC1 Recombinant Antibody (n152b/11).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-VDAC1 Recombinant Antibody (n152b/11) (HB031013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-VDAC1 Recombinant Antibody (n152b/11) (HB031013)

概述

图片

参考文献

产品说明书

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