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Anti-Human HRAS Antibody (Y13-259) (HF778023)

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规格:
  • 50ug
  • 100ug
  • 1mg
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概述
货号HF778023
品牌abinScience
种属反应性Human
应用IF, IP, WB
宿主Mouse
同种型IgG2a, kappa
克隆号Y13-259
克隆类型Monoclonal
靶标c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P01112
状态Liquid
保存溶液 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Human HRAS Antibody (Y13-259).

  • Flow CytoMetry

    Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human HRAS monoclonal antibody (Catalog: HF778023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1μg/ml on various samples.
    Lane 1: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 50 ng
    Lane 2: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 30 ng

参考文献

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