Anti-Human HRAS Recombinant Antibody (Y13-259) (HF778023)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HF778023

abinScience

种属反应性

Human

IF, IP, WB

IgG2a, kappa

Y13-259

c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P01112

Anti-Human HRAS Antibody (Y13-259) (abinScience Cat# HF778023, RRID:AB_3728964)
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Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

GTPase HRas is a ~21 kDa protein. Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

1. Gripp, KW. et al. (2012) American journal of medical genetics. Part A 158A, 2106-18. PMID: 22821884

2. Williams, JG. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 6376-81. PMID: 12740440

3. Guil, S. et al. (2003) Cancer research 63, 5178-87. PMID: 14500341

4. Lander, HM. et al. (1997) The Journal of biological chemistry 272, 4323-6. PMID: 9020151

For research use only.

Isotype Control

Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)

SDS-PAGE

SDS-PAGE for Anti-Human HRAS Antibody (Y13-259).
Flow CytoMetry

Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human HRAS monoclonal antibody (Catalog: HF778023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 50 ng
Lane 2: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 30 ng

产品说明书

Anti-Human HRAS Recombinant Antibody (Y13-259).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human HRAS Recombinant Antibody (Y13-259) (HF778023)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human HRAS Recombinant Antibody (Y13-259) (HF778023)

概述

图片

参考文献

产品说明书

相关产品推荐

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