Anti-Human DNMT3A IP Recombinant Antibody (SAA0199) (HV233013)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HV233013

abinScience

种属反应性

Human

ELISA, FCM, IF, IP

IgG2a

SAA0199

Dnmt3a, DNA MTase HsaIIIA, DNMT3A, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

Q9Y6K1

Anti-Human DNMT3A IP Antibody (SAA0199) (abinScience Cat# HV233013, RRID:AB_3729733)

Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

DNA (cytosine-5)-methyltransferase 3A (DNMT3A) is a ~101 kDa protein. Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting.

1. Chen, T. et al. (2002) The Journal of biological chemistry 277, 38746-54. PMID: 12138111

2. Viré, E. et al. (2006) Nature 439, 871-4. PMID: 16357870

3. Heyn, P. et al. (2019) Nature genetics 51, 96-105. PMID: 30478443

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Flow Cytometry

Flow-cytometry using anti-human DNMT3A antibody.293T cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human DNMT3A antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human DNMT3A antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human DNMT3A antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human DNMT3A IP Antibody (SAA0199).
Flow CytoMetry

Flow-cytometry using FITC anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human DNMT3A monoclonal antibody (Catalog: HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human DNMT3A monoclonal antibody (Catalog: HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human DNMT3A IP Recombinant Antibody (SAA0199).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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实验相关试剂

Anti-Human DNMT3A IP Recombinant Antibody (SAA0199) (HV233013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human DNMT3A IP Recombinant Antibody (SAA0199) (HV233013)

概述

图片

参考文献

产品说明书

相关产品推荐

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